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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
03/01/2019 |
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Actualizado : |
11/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GARMENDIA, G.; PATTARINO, L.; NEGRIN, C.; MARTÍNEZ-SILVEIRA, A.; PEREYRA, S.; WARD, T.J.; VERO, S. |
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Afiliación : |
GABRIELA GARMENDIA, Área Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, General Flores 2124, 11800, Montevideo, Uruguay.; LUCIANA PATTARINO, Área Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay.; CAMILA NEGRIN TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.Área Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay.; ADALGISA MARTÍNEZ-SILVEIRA, Área Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay.; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; TODD J. WARD, Mycotoxin Prevention and Applied Microbiology, USDA-ARS, 1815 N, University St.Peoria, IL, 61604, USA.; SILVANA VERO, Área Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay. |
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Título : |
Species composition, toxigenic potential and aggressiveness of Fusarium isolates causing Head Blight of barley in Uruguay. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Food Microbiology, v. 76, December 2018, p. 426-433. |
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ISSN : |
1095-9998 |
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DOI : |
10.1016/j.fm.2018.07.005 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 22 September 2017// Revised 14 March 2018//Accepted 12 July 2018// Available online 17 July 2018. |
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Contenido : |
ABSTRACT:Fusarium Head Blight (FHB) is a major constraint to barley production that substantially reduces yield and grain quality. FHB is also a major food safety concern because FHB pathogens contaminate grain with trichothecenes and other mycotoxins. DNA sequence-based analyses and in-vitro toxin assessments were used to characterize the species and trichothecene chemotype composition of FHB pathogens on barley in Uruguay. F. graminearum was the dominant species (89.7%), and three other members of the F. graminearum species complex (FGSC) were identified as FHB pathogens of barley in Uruguay for the first time. Other minor contributors to FHB species diversity included F. poae, F. avenaceum, F. pseudograminearum and an unnamed species from the F. incarnatumequiseti species complex (FIESC). Most isolates (89.7%) had the 15-acetyldeoxynivalenol (15-ADON) trichothecene type. However, the results expanded the known area of occurrence within Uruguay for the nivalenol (NIV) toxin type, which was observed among isolates from three species of the FGSC, F. pseudograminearum, and F. poae. Isolates with the 3-acetyldeoxynivalenol (3-ADON) or NX-2 toxin types were not observed, although a previously published multilocus genotyping assay was updated to identify NX-2 strains. Analyses of population structure and comparisons with FHB isolates from wheat in Uruguay indicated that F. graminearum constitutes a single genetic population with no evidence of population differentiation related to the sampled hosts. Inter and intraspecific differences were observed in aggressiveness toward four barley genotypes with different levels of resistance to FHB, and in general nivalenol producers were the least aggressive isolates. Sensitivity to metconazole was approximately 10 times higher than was detected for tebuconazole. This is the first report regarding tebuconazole and metconazole sensitivity for Fusarium species causing FHB in barley in Uruguay, and constitutes an important starting point for monitoring temporal or spatial changes in FGSC sensitivity, which is critical to define FHB management practices. MenosABSTRACT:Fusarium Head Blight (FHB) is a major constraint to barley production that substantially reduces yield and grain quality. FHB is also a major food safety concern because FHB pathogens contaminate grain with trichothecenes and other mycotoxins. DNA sequence-based analyses and in-vitro toxin assessments were used to characterize the species and trichothecene chemotype composition of FHB pathogens on barley in Uruguay. F. graminearum was the dominant species (89.7%), and three other members of the F. graminearum species complex (FGSC) were identified as FHB pathogens of barley in Uruguay for the first time. Other minor contributors to FHB species diversity included F. poae, F. avenaceum, F. pseudograminearum and an unnamed species from the F. incarnatumequiseti species complex (FIESC). Most isolates (89.7%) had the 15-acetyldeoxynivalenol (15-ADON) trichothecene type. However, the results expanded the known area of occurrence within Uruguay for the nivalenol (NIV) toxin type, which was observed among isolates from three species of the FGSC, F. pseudograminearum, and F. poae. Isolates with the 3-acetyldeoxynivalenol (3-ADON) or NX-2 toxin types were not observed, although a previously published multilocus genotyping assay was updated to identify NX-2 strains. Analyses of population structure and comparisons with FHB isolates from wheat in Uruguay indicated that F. graminearum constitutes a single genetic population with no evidence of population differentiation related ... Presentar Todo |
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Palabras claves : |
BARLEY; MYCOTOXINS. |
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Thesagro : |
CEBADA; FUSARIUM; MICOTOXINAS. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 03057naa a2200289 a 4500
001 1059407
005 2019-11-11
008 2018 bl uuuu u00u1 u #d
022 $a1095-9998
024 7 $a10.1016/j.fm.2018.07.005$2DOI
100 1 $aGARMENDIA, G.
245 $aSpecies composition, toxigenic potential and aggressiveness of Fusarium isolates causing Head Blight of barley in Uruguay.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 22 September 2017// Revised 14 March 2018//Accepted 12 July 2018// Available online 17 July 2018.
520 $aABSTRACT:Fusarium Head Blight (FHB) is a major constraint to barley production that substantially reduces yield and grain quality. FHB is also a major food safety concern because FHB pathogens contaminate grain with trichothecenes and other mycotoxins. DNA sequence-based analyses and in-vitro toxin assessments were used to characterize the species and trichothecene chemotype composition of FHB pathogens on barley in Uruguay. F. graminearum was the dominant species (89.7%), and three other members of the F. graminearum species complex (FGSC) were identified as FHB pathogens of barley in Uruguay for the first time. Other minor contributors to FHB species diversity included F. poae, F. avenaceum, F. pseudograminearum and an unnamed species from the F. incarnatumequiseti species complex (FIESC). Most isolates (89.7%) had the 15-acetyldeoxynivalenol (15-ADON) trichothecene type. However, the results expanded the known area of occurrence within Uruguay for the nivalenol (NIV) toxin type, which was observed among isolates from three species of the FGSC, F. pseudograminearum, and F. poae. Isolates with the 3-acetyldeoxynivalenol (3-ADON) or NX-2 toxin types were not observed, although a previously published multilocus genotyping assay was updated to identify NX-2 strains. Analyses of population structure and comparisons with FHB isolates from wheat in Uruguay indicated that F. graminearum constitutes a single genetic population with no evidence of population differentiation related to the sampled hosts. Inter and intraspecific differences were observed in aggressiveness toward four barley genotypes with different levels of resistance to FHB, and in general nivalenol producers were the least aggressive isolates. Sensitivity to metconazole was approximately 10 times higher than was detected for tebuconazole. This is the first report regarding tebuconazole and metconazole sensitivity for Fusarium species causing FHB in barley in Uruguay, and constitutes an important starting point for monitoring temporal or spatial changes in FGSC sensitivity, which is critical to define FHB management practices.
650 $aCEBADA
650 $aFUSARIUM
650 $aMICOTOXINAS
653 $aBARLEY
653 $aMYCOTOXINS
700 1 $aPATTARINO, L.
700 1 $aNEGRIN, C.
700 1 $aMARTÍNEZ-SILVEIRA, A.
700 1 $aPEREYRA, S.
700 1 $aWARD, T.J.
700 1 $aVERO, S.
773 $tFood Microbiology$gv. 76, December 2018, p. 426-433.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha actual : |
05/11/2014 |
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Actualizado : |
11/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 1 |
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Autor : |
AGUERRE, M.; REPETTO, J.L.; PÉREZ-RUCHEL, A.; MENDOZA, A.; PINACCHIO, G.; CAJARVILLE, C. |
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Afiliación : |
JOSE LUIS REPETTO CAPELLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALEJANDRO FRANCISCO MENDOZA AGUIAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Rumen pH and NH3-N concentration of sheep fed temperate pastures and supplemented with sorghum grain. |
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Fecha de publicación : |
2009 |
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Fuente / Imprenta : |
South African Journal of Animal Science, v.39, n.5., p. 246-250, 2009. |
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ISSN : |
0375-1589 |
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DOI : |
10.4314/sajas.v39i1.61157 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT:
The aim of this study was to evaluate the effect of sorghum grain supplementation on ruminal pH and NH3-N concentration of wethers consuming a fresh temperate pasture (Lotus corniculatus) in metabolism cages. Sixteen Corriedale x Milchschaf wethers were fed temperate pastures ad libitum and were non-supplemented or supplemented with ground sorghum grain at 5, 10 or 15 g/kg of their body weight (BW). Rumen fluid samples were collected at 0, 1, 2, 3, 4, 5 and 6 h after supplementation through permanent tubes inserted in the rumen. Ruminal pH was measured immediately and NH3-N concentration was determined by direct distillation. Mean daily pH values for non-supplemented wethers and supplemented with 5, 10 and 15 g/kg of their BW were 6.45, 6.14, 6.09 and 5.43, respectively. Significant differences in pH were found between the 15 g/kg supplemented group and non-supplemented, 5 and 10 g/kg supplemented groups, while a trend was found between non-supplemented and 10 g/kg supplemented group. After 0 h, all mean pH values for the non-supplemented group were above 6.15, while values for the 10 and 15 g/kg supplemented groups were below 6.2 and 6.0, respectively. No differences in NH3-N concentration among groups (mean = 37.15 mg/100 mL), between time or interaction between time and treatment were found. There was a correlation between pH and NH3-N when all measurements were considered. Ground sorghum grain supplementation significantly reduced rumen pH when 15 g/kg of BW was provided to wethers fed temperate pastures, but it did not affect NH3-N concentration. MenosABSTRACT:
The aim of this study was to evaluate the effect of sorghum grain supplementation on ruminal pH and NH3-N concentration of wethers consuming a fresh temperate pasture (Lotus corniculatus) in metabolism cages. Sixteen Corriedale x Milchschaf wethers were fed temperate pastures ad libitum and were non-supplemented or supplemented with ground sorghum grain at 5, 10 or 15 g/kg of their body weight (BW). Rumen fluid samples were collected at 0, 1, 2, 3, 4, 5 and 6 h after supplementation through permanent tubes inserted in the rumen. Ruminal pH was measured immediately and NH3-N concentration was determined by direct distillation. Mean daily pH values for non-supplemented wethers and supplemented with 5, 10 and 15 g/kg of their BW were 6.45, 6.14, 6.09 and 5.43, respectively. Significant differences in pH were found between the 15 g/kg supplemented group and non-supplemented, 5 and 10 g/kg supplemented groups, while a trend was found between non-supplemented and 10 g/kg supplemented group. After 0 h, all mean pH values for the non-supplemented group were above 6.15, while values for the 10 and 15 g/kg supplemented groups were below 6.2 and 6.0, respectively. No differences in NH3-N concentration among groups (mean = 37.15 mg/100 mL), between time or interaction between time and treatment were found. There was a correlation between pH and NH3-N when all measurements were considered. Ground sorghum grain supplementation significantly reduced rumen pH when 15 g/kg of BW wa... Presentar Todo |
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Palabras claves : |
AMBIENTE RUMINAL; GRAIN SUPPLEMENTATION; GRANO DE SORGO; NUTRICIÓN DE RUMIANTES; RUMEN ENVIRONMENT; TEMPERATE PASTURE; WETHER. |
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Thesagro : |
LOTUS CORNICULATUS; NUTRICIÓN ANIMAL. |
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Asunto categoría : |
L51 Fisiología Animal - Nutrición |
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Marc : |
LEADER 02531naa a2200313 a 4500
001 1051524
005 2019-10-11
008 2009 bl uuuu u00u1 u #d
022 $a0375-1589
024 7 $a10.4314/sajas.v39i1.61157$2DOI
100 1 $aAGUERRE, M.
245 $aRumen pH and NH3-N concentration of sheep fed temperate pastures and supplemented with sorghum grain.$h[electronic resource]
260 $c2009
520 $aABSTRACT: The aim of this study was to evaluate the effect of sorghum grain supplementation on ruminal pH and NH3-N concentration of wethers consuming a fresh temperate pasture (Lotus corniculatus) in metabolism cages. Sixteen Corriedale x Milchschaf wethers were fed temperate pastures ad libitum and were non-supplemented or supplemented with ground sorghum grain at 5, 10 or 15 g/kg of their body weight (BW). Rumen fluid samples were collected at 0, 1, 2, 3, 4, 5 and 6 h after supplementation through permanent tubes inserted in the rumen. Ruminal pH was measured immediately and NH3-N concentration was determined by direct distillation. Mean daily pH values for non-supplemented wethers and supplemented with 5, 10 and 15 g/kg of their BW were 6.45, 6.14, 6.09 and 5.43, respectively. Significant differences in pH were found between the 15 g/kg supplemented group and non-supplemented, 5 and 10 g/kg supplemented groups, while a trend was found between non-supplemented and 10 g/kg supplemented group. After 0 h, all mean pH values for the non-supplemented group were above 6.15, while values for the 10 and 15 g/kg supplemented groups were below 6.2 and 6.0, respectively. No differences in NH3-N concentration among groups (mean = 37.15 mg/100 mL), between time or interaction between time and treatment were found. There was a correlation between pH and NH3-N when all measurements were considered. Ground sorghum grain supplementation significantly reduced rumen pH when 15 g/kg of BW was provided to wethers fed temperate pastures, but it did not affect NH3-N concentration.
650 $aLOTUS CORNICULATUS
650 $aNUTRICIÓN ANIMAL
653 $aAMBIENTE RUMINAL
653 $aGRAIN SUPPLEMENTATION
653 $aGRANO DE SORGO
653 $aNUTRICIÓN DE RUMIANTES
653 $aRUMEN ENVIRONMENT
653 $aTEMPERATE PASTURE
653 $aWETHER
700 1 $aREPETTO, J.L.
700 1 $aPÉREZ-RUCHEL, A.
700 1 $aMENDOZA, A.
700 1 $aPINACCHIO, G.
700 1 $aCAJARVILLE, C.
773 $tSouth African Journal of Animal Science$gv.39, n.5., p. 246-250, 2009.
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